Web13 mar 2024 · gDNA和cDNA同属于DNA，都能够被常用的DNA消解酶DNase I消解。. 因此，在逆转录中去除gDNA，一般会通过两步法，即先用DNase I处理RNA模板，之后再将含有DNase I的处理产物进行85℃热失活，之后再进行逆转录，这样可以保证反转录产物cDNA不受到消解。. 但是这种方法操作 ... Web10X DNase I Buffer 5 μl Recombinant DNase I (RNase-free) 2 μl (10 U) Ribonuclease Inhibitor 20 U DEPC-treated water up to 50 μl 2. Incubate for 20 - 30 min at 37℃. 3. Perform one of the following procedures to inactivate DNase I. A．Heat treatment (1) Add 2.5 μl of 0.5 M EDTA, and incubate at 80℃ for 2 min.
DNase I 溶液 (2500 U/mL) - Thermo Fisher
Web1X DNase I Reaction Buffer 10 mM Tris-HCl 2.5 mM MgCl 2 0.5 mM CaCl 2 (pH 7.6 @ 25°C) Usage Concentration 2,000 units/ml Storage Buffer. 10 mM Tris-HCl 2 mM CaCl 2 50% Glycerol pH 7.6 @ 25°C . Heat Inactivation 75°C for 10 minutes Advantages and Features. Application Features. WebDNase I (RNase Free) 本酶是将单链或双链DNA同等程度的随机分解，生成具有 5′-P末端寡核苷酸的脱氧核糖核酸内切酶。. 在 Mg2+存在时， 能在双链DNA上随机地产生切口； … mac キャノン プリンター 設定
Takara官网 - 宝日医生物技术（北京）有限公司
Web10X DNase I Buffer 1 ml ... Takara Bio Inc. 2270A Page 1 of 2 v202404w----Notice To Purchaser: Product Documents, Patent Markings, Trademarks, & Safety Information: Licence Statements: All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. WebSpecifications of Micrococcal Nuclease (Part No. 88216): • Visual: Clear, colorless liquid free of insoluble material. • Units: ≥100 units/µL. • Additional Information: 1 unit is defined as the amount of enzyme required to produce an increase in absorbance at 260nm of 0.001/min/mL at 25°C of highly polymerized DNA. Web翌圣生物提供生产和销售分子生物学工具酶产品:核酸提取与纯化,cdna合成,pcr,qpcr,分子克隆,基因表达,荧光试剂盒等3000多种试剂及试剂盒,现有科研客户数量多达1.2万家。 mac かな 半角