WebThe Huh7 cells were cultured in DMEM media with 10% FBS and I have added antibiotic and antimycotic. These are appearing in the culture plate 2days after seeding cells and addition of extra anti...
Sphere-forming culture enriches liver cancer stem cells and …
WebThe majority of Huh-7 cells show a chromosome number between 55 and 63 (mode 60) and are highly heterogeneous. These cells are adherent to the surface of flasks or plates and … WebApr 12, 2024 · Lysosomal homeostasis and membrane maintenance involve ESCRT-dependent mechanisms, with protein ALIX playing a major role in lysosomal membrane repair. 44, 47, 77, 78 Thus, we tested whether ATG5 has an effect on ALIX recruitment to damaged lysosomes. In Huh7 ATG5-WT cells, ALIX puncta were induced and ALIX co … helps initial digestion in the mouth
Liver cancer cell lines distinctly mimic the metabolic gene …
WebJul 16, 2024 · Huh7-IR ( a) and Huh-CR ( b) cells were treated with 5 µM MG132 and 20 µM DFO for 8 h before harvest to enrich for HIF1α protein. anti-HIF1α antibody and irrelevant anti-IgG as control were used... WebAug 20, 2012 · Curcumin or DMSO was added to the medium for 24 h. Total RNA was isolated and analyzed by real-time RT-PCR. (C) Curcumin inhibits HCV protein expression in Huh7.5-HCV cells. Cells were subcultured at a density of 1.5×10 6 cells in 8 ml of culture medium in a 10-cm plastic dish for 6 h. Curcumin or DMSO was added to the medium … WebAug 1, 2024 · Two HCC cell lines (Huh7 and Hep3B) were cultured in ultra-low attachment surface plates with serum-free medium, and both cell lines formed sphere clusters. As drug resistance is a main characteristic of CSCs, we treated sphere-forming cells with 5-FU, Sorafenib, or Doxorubicin to evaluate drug resistance. l and c ties